Production of glucose isomerase
AssigneeImperial Chemical Industries PLC
InventorJohn Laurence Meers
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Abstract
A process for the continuous production of glucose isomerase by maintaining a nutrient in the culture medium at a concentration which is limiting to growth. Preferably the growth limiting nutrient is the carbon source. The invention also covers a process for isomerizing glucose to fructose using continuously prepared glucose isomerase. Important conditions for culturing the glucose isomerase producing microorganism are the dissolved oxygen tension and dilution rate.
Claims15
A process for producing glucose isomerase enzyme by continuously cultivating a glucose isomerase-producing microorganism in a culture medium comprising a source of assimilable carbon and inorganic nutrients under conditions suitable for production of said enzyme and continuously recovering said enzyme wherein the concentration of a nutrient source in the culture medium is maintained at a level such as to be limiting to growth.
A process which comprises isomerizing glucose to fructose wherein there is added in said isomerizing a glucose isomerase enzyme which has been produced by continuously cultivating a glucose isomerase-producing microorganism in a culture medium comprising a source of assimilable carbon and inorganic nutrients under conditions suitable for production of said enzyme and continuously recovering said enzyme wherein the concentration of a nutrient source in the culture medium is maintained at a level such as to be limiting to growth.
Dependencies are inferred from the claim text.