Purine nucleoside 5'-phosphate (mono, di or tri) 3'(2')-diphosphates and processes for their preparation
AssigneeSanraku-Ocean Co., Ltd.
InventorsToyokazu Nishino, Yasutaro Hamagishi, Toshikazu Oki, Sawao Murao
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Abstract
New purine nucleoside 5'-phosphate (mono, di or tri) 3'(2')-diphosphates and the sodium, lithium and potassium salts thereof are elaborated by the enzymatic transfer of pyrophosphoryl group from ATP (adenosine triphosphate), dATP (deoxyadenosine triphosphate) and pppApp (adenosine 5'-triphosphate 3'(2')diphosphate) to specified 5'-purine nucleotides, and a new nucleotide pyrophosphotransferase used for this enzyme reaction is produced by actinomycetes and other microorganisms and recovered from the culture filtrate and mycelium by the conventional methods for recovering enzymes.
Claims6
A process for producing the purine nucleoside 5'-phosphate (mono, di or tri) 3'(2')-diphosphates of the formula mXpp,
- wherein m represents one to three phosphoryl groups constituted by at least one of phosphate residues and methylene diphosphate residues, each p represents a phosphate residue and X represents a nucleoside selected from the group consisting of adenosine, guanosine and inosine, or lithium, potassium or sodium salts thereof which comprises placing a donor nucleotide or deoxynucleotide and an acceptor nucleotide in contact with a pyrophosphotransferase produced by an actinomycetes belonging to the genera Streptomyces, Streptoverticillium or Actinomyces, and characterized by a substrate specificity for a nucleotide or a deoxynucleotide as a donor and a nucleotide as an acceptor and the capability of transferring a pyrophosphoryl group from the 5'-position of said donor nucleotide or deoxynucleotide to the 3'(2') position of said acceptor nucleotide, incubating the resultant reaction mixture to effect the transfer of a pyrophosphoryl group of the 50'-position of said donor nucleotide or deoxynucleotide to the 3'(2')-position of said acceptor nucleotide and recovering the thus obtained purine nucleoside 5'-phosphate(mono, di or tri) 3'(2')-diphosphate or salts thereof.
Dependencies are inferred from the claim text.