Cell culture process
AssigneeMonsanto Company
InventorWilliam R. Tolbert
Life of this patent
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Abstract
SV3T3 cells are rapidly propagated in vitro to elaborate MIF and TAF by first growing on a surface and thereafter transferring an inoculum of the growing cells into an agitated liquid suspension of nutrient culture medium, incubating at 30.degree.-38.degree. C while continuing the agitation of the medium, periodically transferring the growing cells into fresh medium and continuing the incubation with agitation of the medium, whereby said cells are proliferated in aggregates and elaborate MIF and TAF in significantly detectable amounts.
Claims10
A method of rapidly propagating SV3T3 cells in vitro to elaborate MIF and TAF comprising growing SV3T3 cells on a surface, thereafter transferring an inoculum of said growing cells into a normally liquid suspension of nutrient culture medium of known essential amino acids, mineral salts, vitamins, and carbohydrates, incubating at a temperature of from about 30.degree. to about 38.degree. C while substantially continuously maintaining agitation of said medium, periodically transferring the growing cells into fresh nutrient culture medium and continuing the agitated incubation until harvest whereby said cells are proliferated in aggregates and elaborate MIF and TAF in significantly detectable amounts.
Dependencies are inferred from the claim text.