Cytomegalovirus attenuation method and vaccine
OwnerHarold Stern, Stephen Dyonis Elekinventors
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Abstract
An attenuated strain of cytomegalovirus is prepared by serial passage in human tissue culture cells. The stain of virus so attenuated is incorporated into vaccines and used for developing immunity in humans against infections with cytomegalovirus.
Claims16
A method of preparing an attenuated strain of cytomegalovirus comprising serially passaging at least 40 times a cytomegalovirus isolate in susceptible human fibroblast cell cultures to obtain a virus strain which stimulates the production of complement fixing and neutralising antibodies in susceptible humans, when tested about 10 weeks after the subcutaneous administration of 10.sup.4 T.C.D..sub.50 units of the strain, without any significant side effects lasting longer than 1 to 2 weeks, or detectable excretions of the virus from the test subjects.
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