Chemically modified endotoxin immunizing agent
AssigneeAbbott Laboratories
InventorFloyd Cottam McIntire
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Abstract
A bacterial endotoxin lipopolysaccharide of reduced toxicity covalently coupled to a protein antigen exhibits enhanced adjuvant effects. The coupling was affected by reaction with haloacylhalide. Lipopolysaccharide acylated with an anhydride of a dibasic acid is detoxified; in combination with endotoxin polysaccharide covalently coupled to protein antigen it developed synergistic immunogenic effects.
Claims2
A bacterial endotoxin derivative of reduced toxicity comprising a gram-negative bacterial lipoplysaccharide having at least one free amino group and an anhydride selected from the group consisting of compounds of the formula ##STR5## wherein n is 0 or 1, and R, R.sub.1 and R.sub.2 each represent hydrogen, C.sub.1 -C.sub.6 alkyl, C.sub.2 -C.sub.6 alkenyl, C.sub.5 -C.sub.6 cycloalkyl, phthalyl or methylphthalyl wherein any two R groups taken together form a saturated cyclic substituent.
Dependencies are inferred from the claim text.