Immunity depressant medicine
OwnerMarc Bonneau, Mireille Latourinventors
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Abstract
Immunity-depressing biologic medicine comprising in serum an effective amount of immuno-depressive gamma-globulins obtained by elution of ground and washed human placental tissue with an acid buffer and separation from the eluate of the thus-freed gamma-globulins by precipitation.
Claims16
A process for extracting immuno-depressive gamma-globulins from human placental tissue which has been ground or powdered comprising the following steps:
- a. Washing the ground placental tissue to remove serous substances therefrom;
- b. Eluting the placental tissues, freed of serous substances, with a buffer solution to form an eluate;
- and freeing the eluate of any tissue material and precipitates wherein the elution of the placental tissue is undertaken at a pH of about between 2 and 3.5 with said buffer solution and at a temperature ranging between about 10.degree. to 38.degree. C;
- c. Adding ethanol to said eluate to precipitate the immuno-depressive gamma-globulins;
- and
- d. Dialyzing the immuno-depressive gamma-globulins with water.
A process for extracting immuno-depressive gamma-globulins from human placental tissue which has been ground or powdered comprising the steps of:
- a. Washing the ground placental tissue to remove serous substances therefrom;
- b. Eluting the placental tissues, freed of serous substances, with a buffer solution having an acid pH of 6.5 to a basic pH of 7.5 to form an eluate;
- and freeing the eluate of any tissue material and precipitate;
- c. Adding ethanol to said eluate to precipitate the immuno-depressive gamma-globulins;
- and
- d. Dialyzing the immuno-depressive gamma-globulins with water.
A process for extracting immuno-depressive gamma-globulins from human placental tissue which has been ground or powdered comprising the following steps:
- a. Washing the ground placental tissue to remove serous substances therefrom;
- b. Eluting the placental tissues, freed of serous substances, with a buffer solution to form an eluate;
- and freeing the eluate of any tissue material and precipitates wherein the elution of the placental tissue is undertaken with said buffer solution having an acid pH and at a temperature ranging between about 10.degree. to 38.degree. C;
- c. Adding ethanol to said eluate to precipitate the immuno-depressive gamma-globulins;
- and
- d. Dialyzing the immuno-depressive gamma-globulins with water.
A process for extracting immuno-depressive gamma-globulins from human placental tissue which has been ground or powdered comprising the following steps:
- a. Washing the ground placental tissue to remove serous substances therefrom;
- b. Eluting the placental tissues, freed of serous substances, with a buffer solution to form an eluate;
- and freeing the eluate of any tissue material and precipitates wherein the elution of the placental tissue is undertaken with said buffer solution which has a substantially neutral pH and at a temperature ranging between about 10.degree. to 38.degree. C;
- c. Adding ethanol to said eluate to precipitate the immuno-depressive gamma-globulins;
- and
- d. Dialyzing the immuno-depressive gamma-globulins with water.
Dependencies are inferred from the claim text.