Cardiac glycoside or aglycone assays
AssigneeSyva Company
InventorsEdwin F. Ullman, Joyce Y. Chang, Kenneth G. McNeil
Life of this patent
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Abstract
Immunoassays for digoxin and its congeners are provided by pre-treating the serum sample with dilute aqueous alkaline solutions under mild conditions and for short periods of time, preferably in combination with a chelating agent, prior to carrying out the immunoassay. The method finds particular application with radioimmunoassays and homogeneous enzyme immunoassays.
Claims9
In an immunoassay for the determination of cardiac glycosides or aglycones employing a serium sample, wherein antibody for cardiac glycosides or aglycones is employed which binds to cardiac glycosides or aglycones in competition with the indicator used for detection, the improvement which comprises:
- pre-treating said serum sample with an alkaline solution under mild conditions at a pH of at least about 10.5.
Dependencies are inferred from the claim text.