Lipase composition for glycerol ester determination
AssigneeThe Dow Chemical Company
InventorsCharles Y. Huang, Alejo V. Roy
Life of this patent
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Abstract
A synergistic composition of microbial lipases from Rhizopus arrhizirs and Candida cylindracea is used for the hydrolysis of glycerol esters such as triglycerides in the determination of glycerol esters present in an aqueous medium such as body fluid.
Claims13
A composition useful for the hydrolysis of a glycerol ester in an aqueous medium comprising a mixture of from 15 to 95 units of Rhizopus arrhizus lipase and from 5 to 85 units of Candida cylindracea lipase per 100 units of total lipase present.
A reagent composition useful for the determination of triglyceride concentration in serum by measuring the light absorbance of the serum following hydrolysis of the triglycerides to glycerol and fatty acids which comprises an enzyme mixture having from 15 to 95 units of Rhizopus arrhizus lipase and from 5 to 85 units of Candida cylindracea lipase per 100 units of total lipase and chemical means for measurably changing the spectroscopic absorbance of the composition in proportion to the amount of glycerol released in the serum.
A method for hydrolyzing a glycerol ester and for determining the amount of glycerol ester present in an aqueous medium which comprises incubating the glycerol ester at a pH of 5.5-9.0 with a lipase mixture having the proportions of 15 to 95 units of a lipase from Rhizopus arrhizus and 5 to 85 units of a lipase from Candida cylindracea per 100 units of total lipase present for a time sufficient to hydrolyze the glycerol ester and determining the amount of glycerol or fatty acids released by the hydrolysis.
Dependencies are inferred from the claim text.