Purification of microbial enzyme extracts using synthetic polyelectrolytes
AssigneeEastman Kodak Company
InventorsRoy Eugene Snoke, Gerald Wayne Klein
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Abstract
Nucleic acids and unwanted proteins are removed from microbial enzyme extracts by precipitation with water-soluble, cationic polymers comprised of monomers having the structure ##STR1## wherein R.sub.1 is a hydrogen atom, a lower alkyl group, or the equivalent and R.sub.2 is a nitrogen containing group capable of carrying a positive electrical charge when the polymer is dissolved in an aqueous solution.
Claims14
A method for purifying a microbial enzyme extract, comprising the step of precipitating nucleic acids and unwanted protein by adding to the extract an effective amount of a water-soluble, cationic polymer comprised of a cationic monomer having the formula ##STR4## wherein R.sub.1 is a hydrogen atom or a lower alkyl group, and R.sub.2 is a nitrogen containing group capable of carrying a positive electrical charge when the polymer is dissolved in an aqueous solution having a pH value not greater than about the pKa of the polymer.
A method for purifying a microbial enzyme extract, said method comprising the step of precipitating nucleic acids and unwanted protein by adding to the extract an effective amount of a water-soluble, cationic polymer comprised of a cationic monomer having the formula ##STR5## wherein:
- R.sub.1 is a hydrogen atom or a lower alkyl group;
- and
- R.sub.2 is selected from the group consisting of ##STR6## where X is an anion.
A method for purifying a microbial enzyme extract, said method comprising the step of precipitating nucleic acids and unwanted protein by adding to the extract an effective amount of a water-soluble, cationic polymer comprised of a monomer having the formula ##STR7## wherein:
- R.sub.1 is a hydrogen atom or a lower alkyl group;
- and
- R.sub.2 is selected from the group consisting of ##STR8## where X is an anion, said monomer comprising at least 10 percent by weight of said polymer and said polymer being added to the extract in an amount of from about 0.01 to about 0.5 mg per mg of protein in the extract.
Dependencies are inferred from the claim text.