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OwnerNelli Isaakovna Zhdanova, Lev Mikhailovich Evstjugov-Babaev +4inventors
InventorsNelli Isaakovna Zhdanova, Lev Mikhailovich Evstjugov-Babaev, Roza Mitrofanovna Balitskaya, Albert Fedorovich Sholin, Tatyana Borisovna Kasatkina, Natalya Nikolaevna Kuznetsova
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The method of preparing L-glutamic acid and its sodium salt by growing, on a nutrient medium containing sources of nitrogen, carbon and mineral salts a strain producent of L-glutamic acid, Micrococcus glutamicus "VNIIgenetika" 3144, obtained from the strain Micrococcus glutamicus "VNIIGenetika" 490 by multi-step selection with mutagenic factors-diethyl sulphate and ultra-violet radiation-- the biotin concentration in the liquid mineral medium preferably being such as to ensure the maximum yield of L-glutamic acid.
A method of preparing L-glutamic acid and its sodium salt which comprises cultivating on a nutrient medium containing sources of carbon, nitrogen, and mineral salts Micrococcus glutamicus"VNIIGenetika" 3144, obtained from strain Micrococcus glutamicus "VNIIGenetika" 490 by multistep selection with mutagenic factors diethyl sulphate and ultra-violet radiation, precipitating the resulting biomass and recovering L-glutamic acid therefrom.
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