Preparation for treating squamous cell carcinoma and method for making same
AssigneeIntermountain Laboratories, Inc.
InventorGeorge T. Manilla
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Abstract
A biological preparation, method of preparation, and method of treatment of animals as concerns squamous cell carcinoma, known commonly as "Cancer Eye". Used in the preparation of the biological product is tumorous tissue, or culture thereof, obtained from a so-diseased animal. The tumor is introduced into and homogenized in a hypertonic metallic salt solution containing both an adsorbing agent and also a suspending agent. Temperature of the material must not rise during homogenization above 60.degree. C. The homogenized tumor tissue mixture is allowed to stand, with occasional mixing preferred. Certain additives produce useful material which is suitably processed and introduced into an injection medium.
Claims11
A method for preparing a biological preparation for treatment of squamous cell carcinoma, comprising the steps of:
- homogenizing squamous cell carcinoma cells in a one to five molar hypertonic metallic chloride salt solution containing an adsorbing agent and a suspending agent at a temperature below about 60.degree. C.;
- extracting the cells in the suspension for at least about 12 hours at a temperature of at least about 26.degree. C.;
- separating the liquid extract from the solid material to produce a liquid containing the cell extract;
- contacting the liquid containing the cell extract with an aqueous metallic chloride salt solution and a low carbon chain alcohol for at least about 12 hours at a temperature of from about 4.degree. C. to about 25.degree. C. to to strip the cell extract from the liquid and to form a precipitate containing the extract;
- dissolving the precipitate in an organic extraction medium, and allowing the solution to stand for a sufficient amount of time to purify the extract;
- and
- reducing the presence of the extracting medium to precipitate the purified cell extract.
A biological preparation of treatment of squamous cell carcinoma, comprising an extract from squamous cell carcinoma cells prepared by the following steps:
- homogenizing squamous cell carcinoma cells in a one to five molar hypertonic metallic chloride salt solution containing an adsorbing agent and a suspending agent at a temperature below about 60.degree. C.;
- extracting the cells in the suspension for at least about 12 hours at a temperature of at least about 26.degree. C.;
- separating the liquid extract from the solid material to produce a liquid containing the cell extract:
- contacting the liquid containing the cell extract with an aqueous metallic chloride salt solution and a low carbon chain alcohol for at least about 12 hours at a temperature of from about 4.degree. C. to about 25.degree. C. to strip the cell extract from the liquid and to form a precipitate containing the extract;
- dissolving the precipitate in an organic extraction medium, and allowing the solution to stand for a sufficient amount of time to purify the extract;
- and
- reducing the presence of the extracting medium to precipitate the purified cell extract.
Dependencies are inferred from the claim text.