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AssigneeRecherche et Industrie Therapeutiques
InventorsLucia Dobrescu, Constant Huygelen
Estimating the term…
Piglet colibacillosis vaccine for intramuscular or subcutaneous administration to pregnant sows comprising cell-free heat labile (LT) E. coli enterotoxin with an adjuvant and the method for preventing piglet colibacillosis consisting in administering said vaccine to pregnant sows from 3 to 6 weeks before farrowing.
A pre-farrowing pregnant sow piglet colibacillosis vaccine for intramuscular or subcutaneous administration to pregnant sows 3 to 6 weeks before farrowing comprising a unit dosage or multidose integer of the dosage of an effective amount of at least 5 to 150 mg. lipopolysaccharide- and cell-free heat labile (LT) E. coli enterotoxin isolated from a culture of an E. coli serotype which is capable of causing piglet colibacillosis and protective against the actions of heat stable and heat labile enterotoxins produced by heterologous as well as homologous enteropathogenic E. coli serotypes, and an effective amount of an adjuvant selected from the group consisting of aluminium hydroxide, aluminium phosphate, peanut oil emulsified by the addition of monooleate and stabilized by the addition of aluminium monostearate or Freund's adjuvant.
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Enterobacteriales, e.g. Enterobacter: escherichia
Medicinal preparations containing antigens or antibodies: characterised by the dose, timing or administration schedule
Characterised by the host/recipient, e.g. newborn with maternal antibodies: veterinary vaccine
In human health protection, e.g. against extreme weather: against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne…
Term ended 11 Oct 1994 (estimated)
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This invention relates to new colibacillosis vaccines, more particularly vaccines against piglet colibacillosis.
Neonatal colibacillosis of swine is an important cause of death in piglets and numerous attempts have been made to control this illness by actively immunizing the dam against Escherichia coli (E. coli) either by killed or live vaccines or by giving the pigs E. coli antiserum.
Active immunization of the sows has been performed by parenteral administration of E. coli vaccines (see for instance M.R. Wilson and J. Svendsen. Amer. J. Vet. Res. 32, 1971, 891-898) but because the E. coli strain or strains used for immunization may only generate specific antibodies while there may be several and different strains associated with a given outbreak, these attempts at prophylaxis based on antibacterial immunity have given inconsistent and generally unsatisfactory results. Even if in some cases good protection was obtained, it was restricted to those serotypes of E. coli present in the vaccine.
Water (10 liters) is added to 130 g. of BACTOTRYPTOSE broth and 150 g. of BACTO-AGAR (BACTO-TRYPTOSE and BACTO-AGAR are trade names of products manufactured and sold by DIFCO Laboratories, Detroit 1, Mich., U.S.A.). The mixture is heated with stirring up to 121.degree. C. for 60 minutes. Twenty g. of BACTO-DEXTROSE (trade name of a product manufactured and sold by DIFCO Laboratories) are dissolved in 20 ml. of distilled water and the solution is passed through a 0.45 micron Millipore sterilizing filter (Millipore Corporation, Bedford, Mass., U.S.A.). Both preparations are then pooled and 100 ml. aliquots are distributed into 500 square centimeter Roux flasks.
E. coli strain ATCC 21972 is re-hydrated with sterile saline and incubated for 18 hours at 37.degree. C. in Petri dishes containing each 20 ml of TRYPTOSE-AGAR solid medium prepared by mixing 26 g. of TRYPTOSE broth, 30 g. of AGAR DIFCO (TRYPTOSE broth and AGAR DIFCO are products manufactured and sold by DIFCO Laboratories) up to one liter with water, the mixture being heated for 45 minutes at 115.degree. C.
A liquid culture medium (named PP.sub.3) is then prepared as follows : Proteose peptone No. 3 (a product manufactured and sold by DIFCO Laboratories) 30 g., yeast extract (4 g.) and dextose (5 g.) are dissolved in 1 liter of water at 60.degree. C. After cooling, NaCl (5 g.), NaHPO.sub.4 (5.05 g.) and KH.sub.2 PO.sub.4 (1.2 g.) are added thereto. The medium, the pH of which is 6.9-7.0 is filtered on Seitz EKS filter and distributed into 100 ml. culture flasks.