Process for the fermentation of carbohydrate-containing nutrient substrate in the preparation of citric acid, wherein a fermentation solution is inoculated with a fermentation agent in a fermentation chamber, a layer of the fermentation agent covering the entire surface of the nutrient substrate is formed in a germination phase, and subsequently a fermentation phase is carried out, characterized in that air is pretreated to a highly sterile state and fed into the fermentation chamber during the germination phase in a quantity sufficient to maintain the chamber at a slight positive pressure;
- the fermentation chamber is heated separately and independently of the air supply during the germination phase;
- the feeding of the highly sterile air and the heating of the fermentation chamber are discontinued during the fermentation phase, and fresh, normally filtered, unconditioned air is introduced in a quantity sufficient to maintain the fermentation solution at an optimum fermentation temperature.