Serologic test for systemic candidiasis

In an in vitro test method for the diagnosis of systemic candidiasis in a mammalian host, said test method being based on precipitin formation between antibodies in a blood serum sample from said host and antigens in solution, said antibodies and antigens each diffusing through an agar layer maintained at a pH of 7 to 9.6, to encounter one another and form said precipitin, the improvement which comprises incorporating in said antigen solution a concentration of protein antigens in the range of 0.5 to 10 mg. per ml and a concentration of mannan antigen in the range of 0.025 to 1 mg. per ml, the weight ratio of said protein antigens to mannan being in the range of 5:1 to 10:1 `said protein and mannan antigens prepared from the yeast phase, whereby false readings are reduced.