Preparation of ferrous hemoglobin and enzymatic digestion products thereof active for absorption of carbon monoxide
OwnerIsrael Herbert Scheinberginventor
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Abstract
Ferrous hemoglobin in a form suitable for absorption of carbon monoxide is produced from a hemoglobin solution, buffered to prevent denaturation of the hemoglobin, and containing an effective reductant. The quantity of reductant present is at least sufficient to counteract the effect of oxygen in such air as comes in contact with the hemoglobin. Enzymatic digestion products of hemoglobin, also active for the absorption of carbon monoxide are produced by controlled decomposition of hemoglobin. These products are lower in equivalent weight than is hemoglobin so that the quantity needed for absorbing a given quantity of carbon monoxide is reduced.
Claims21
Method of producing ferrous hemoglobin from an aqueous solution of hemoglobin wherein said solution is to be exposed to a limited quantity of oxygen during production of said ferrous hemoglobin, comprising the steps of adding during agitation of said solution a buffer under conditions such as to maintain said solution at a pH between about 6.0 and 8.5 both locally and overall, and during addition of reductant and adding to said solution a quantity of an effective reductant sufficient to substantially prevent the inactivation of said hemoglobin for the absorption of CO by said limited quantity of oxygen.
Dependencies are inferred from the claim text.