Methods for the preparation of semi-synthetic aminocyclitol aminoglycoside antibiotics

A process for producing Antibiotics Mu-1, Mu-2, Mu-4, Mu-5 and Mu-6 which comprises fermenting Micromonospora inyoensis strain 1550F-1G in an aqueous nutrient medium, adding to the fermentation a compound of the formula:

• ##STR16## wherein A is a member selected from the group consisting of hydrogen, amino, hydroxy and (C.sub.1 -C.sub.8) alkoxy, B is a member selected from the group consisting of hydrogen and hydroxy, wherein the wavy lines connecting A and B to the ring nucleus denote that such groups may be in any of the possible stereoisomeric forms, with the proviso that when B is hydrogen and A is hydroxy, A must be cis to the hydroxyl groups adjacent thereto and with the further proviso that when A is amino, it must be trans to the hydroxy groups adjacent thereto, continuing the fermentation until a composition of matter having substantial antibacterial activity is produced and isolating an antibiotic therefrom.