Method of preparing enzyme cholic acids complex

A method of preparing an enzyme-hormone complex containing between approximately 8 and 12 units/mg of enterokinase, more than 40 units/mg of maltase, more than 10 units/g of alkaline phosphatase, more than 2 units/mg of aminopeptidase and trehalase, glutamyl transferase, ornithine carbamyl transferase, saccharase, lactase, enterogastrone, secretin, cholecystokinin, caerulin and pancreozymin, from the small intestine of pigs or bullocks, comprising the steps of:

• a. mixing pieces of fresh or refrigerated small intestine with an aqueous solution containing between 100 and 600 g of cholic acids constituted by a mixture of cholic acid, desoxycholic acid, glycocholic acid and taurocholic acid and between 50 and 400 ml of polyethylene glycol mono-para (1,1,3,3 tetramethyl butyl)phenyl ether per 100 liters of water, the proportion of said aqueous solution being between about 100 and about 250 liters per 100 kg of small intestine;
• b. maintaining the resulting mixture for about 2 to about 12 hours at a temperature between 0 to 5.degree. C and at a pH of about 6 to 7;
• and
• c. separating from the mixture the treated pieces of small intestine by filtration in order to obtain a solution containing said enzymes and hormones.