Identification of non-fermentative gram-negative bacteria
AssigneeThe United States of America as represented by the Department of Health, Education and Welfare
InventorLucy A. Otto
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Abstract
A method for identification of non-fermentative Gram-negative bacteria (NFB) consisting of correlating results from a plurality of oxidative alkalinization (OAL) assays in media samples consisting essentially of a minimal-protein medium, an indicator and a substrate and a plurality of oxidative acidification (OAC) assays in media consisting essentially of a minimal-protein medium, an indicator and a substrate. A medium for determining oxidative attack (OA) consists essentially of a minimal-protein medium, an indicator and a substrate.
Claims9
Assay medium for determining oxidative attack (OA) by Gram-negative non-fermentative bacteria (NFB) consisting essentially of an oxidative alkalination (OAL) assay medium containing 0.02 -0.05% by weight of casamino acids, bromthymol blue indicator, a substrate and buffered to pH 6.0 and an oxidative acidification (OAC) medium containing 0.02-0.03% by weight of tryptone, bromthymol blue indicator, a substrate and buffered to pH 6.7.
A method for the identification of "an unknown" non-fermentative Gram-negative bacteria (NFB) consisting of testing said unknown by the results of a battery of oxidative alkalination (OAL) assays in media samples consisting essentially of a minimal-protein medium containing 0.02-0.05% by weight of casamino acids and buffered to pH 6.0, bromthymol blue indicator and a substrate and a plurality of oxidative acidification (OAC) assays in media samples consisting essentially of a minimal-protein medium containing 0.02-0.03% by weight of tryptone and buffered to pH 6.7, bromthymol blue indicator and a substrate.
A kit for the identification of non-fermentative Gram-negative bacteria (NFB) consisting of a compartmented tray containing media for oxidative alkalination (OAL) assays and a compartmented tray containing media for oxidative acidification (OAC) assays, wherein each compartment contains minimal-protein medium, bromthymol blue indicator and a different substrate, and wherein minimal-protein media for OAL assays contains 0.02-0.05% by weight of casamino acids and is buffered to pH 6.0 and for OAC asays contains 0.02-0.03% by weight of tryptone and is buffered to pH 6.7.
Dependencies are inferred from the claim text.