Azide bonding of a protein to a latex
AssigneeThe Dow Chemical Company
InventorLinneaus C. Dorman
Life of this patent
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Abstract
A method of coupling a latex having a surface carboxylic amide group to a protein by an amide bond for producing a composition useful in immunological testing for antibodies or antigen.
Claims6
A method for coupling a latex having surface carboxylic amide groups to a protein having free amino groups which comprises hydrazinolysing the carboxylic amide group on the latex to a carboxylic hydrazide group by treatment with hydrazine, diazotizing the carboxylic hydrazide group on the latex with a nitrite in the presence of an acid to form a carboxylic azide group, and coupling the carboxylic azide group of the latex with a free amino group on the protein.
A method for coupling monodispersed styrene acrylamide latex to human chorionic gonadotropin comprising hydrazinolysing the carboxylic amide group on the latex to a carboxylic hydrazide by treatment with hydrazine at a temperature of 40.degree.-60.degree. C., dialyzing the latex for a time sufficient to remove unreacted hydrazine, diazotizing the carboxylic hydrazide group formed on the latex with sodium nitrite and hydrochloric acid, and coupling the human chorionic gonadotropin to the latex at a pH of 7-9.5 at a temperature of 2.degree.-5.degree. C. for a period of at least 24 hours.
Dependencies are inferred from the claim text.