Diazepam enzyme conjugates

An enzyme-bound-benzdiazocycloheptane of the following formula ##STR4## wherein:

• any one of the W groups other than W.sup.a 36 can be --X*;
• X* is a bond or linking group bonded to the enzyme at other than its active site;
• A* is an enzyme;
• n is on the average in the range of from about 1-20;
• when other than X*:
• W.sup.a30 and W.sup.a35 are hydrogen;
• W.sup.a31 is hydrogen, lower alkyl of from 1-3 carbon atoms, or may be taken together with W.sup.a32 to form a double bond between the carbon and the nitrogen;
• W.sup.a33 is amino, lower alkyl amino of from 1-3 carbon atoms or may be taken together with W.sup.a32 to form a carbonyl;
• W.sup.a34 is hydrogen or hydroxyl;
• and
• W.sup.a36 is oxy or an unshared pair of electrons.

An enzyme-bound-benzdiazocycloheptane of the formula ##STR5## wherein:

• anyone of the W groups can be --X*;
• A* is an enzyme;
• n is on the average in the range of 2-12;
• when other than X*:
• W.sup.a31 is hydrogen or lower alkyl of from 1-3 carbon atoms;
• and
• A.sup.a34 is hydrogen or hyroxyl;
• X* is of the formula ##STR6## wherein:
• Y is oxygen or a bond, being a bond when W.sup.a31 is X*;
• Z is an aliphatic group having from 0-1 site of ethylenic unsaturation as its only unsaturation and of from 1-7 carbon atoms and having from 0-1 heteroatom of atomic number 7-8 bonded solely to carbon;
• U is a bond or a non-oxo-carbonyl group including the nitrogen and sulfur analogs thereof;
• and
• p and q are 0 or 1.