Method for detecting enzymes capable of digesting fibrinogen or fibrin
AssigneeCanadian Patents and Development Limited
InventorLeonard Arthur Moroz
Life of this patent
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Abstract
A method for detecting and measuring the activity of enzymes capable of digesting fibrinogen or fibrin and for detecting and measuring the activity of enzyme activators and enzyme inhibitors of said enzymes, by incubating an unknown enzyme sample in a humid labelled fibrinogen or labelled fibrin coated test apparatus, isolating the labelled degradation products of fibrinogen or fibrin released by the unknown enzyme sample and measuring the labelled degradation products of fibrinogen released by the unknown sample.
Claims10
A method for detecting and measuring enzymes capable of digesting fibrinogen and for detecting and measuring activators and inhibitors of enzymes capable of digesting fibrinogen which comprises:
- a. coating at room temperature a water-insoluble polymer test apparatus with a buffered solution of a radioactive tracer labelled enzyme-free human fibrinogen to produce a moist coating;
- b. reacting said moist coating with a thrombin solution thereby converting the labelled fibrinogen to labelled fibrin;
- c. contacting said coating of radioactively labelled fibrin with a sample of the unknown enzyme or a mixture of a known enzyme capable of digesting fibrin and the unknown enzyme activator or a mixture of a known enzyme capable of digesting fibrin and the unknown enzyme inhibitor;
- d. incubating said sample in contact with said coating;
- e. washing the test apparatus with a buffered solution and recovering the solution containing the radioactively digested products released by said sample;
- f. counting radiation emitted by said digested and released fibrin and comparing said counts with the number of counts from the coated test apparatus prepared in Step (a).
A method for detecting and measuring enzymes capable of digesting fibrinogen and for detecting and measuring activators and inhibitors of enzymes capable of digesting fibrinogen which comprises:
- a. coating at room temperature a water-insoluble polymer test apparatus with a buffered solution of a radioactive tracer labelled enzyme-free human fibrinogen to produce a moist coating;
- b. contacting said moist coating of radioactively labelled fibrinogen with a sample of the unknown enzyme or a mixture of a known enzyme capable of digesting fibrinogen and the unknown enzyme activator or a mixture of a known enzyme capable of digesting fibrinogen and the unknown enzyme inhibitor;
- c. incubating said sample in contact with said coating;
- d. washing the test apparatus with a buffered solution and recovering the solution containing the radioactive digested products released by said sample;
- e. counting radiation emitted by said digested and released products of fibrinogen and comparing said counts with the number of counts from the coated test apparatus prepared in Step (a).
Dependencies are inferred from the claim text.