Diagnostic method and compounds for use therewith
AssigneePrinceton Biomedix Incorporated
InventorsGeorge F. Bulbenko, Joseph Eliot Woodbridge
Life of this patent
Estimating the term…
Abstract
An assay for phosphatase enzymes in body fluids comprising contacting a sample of such body fluid with the substrate, Thymol Blue Monophosphate, which is chromogenic at the pH of the reaction mixture and determining activity of the enzyme by measuring the rate of formation of the Thymol Blue dye.
Claims9
Compounds useful in the assay of alkaline phosphatase enzymes comprising a compound having the structure ##STR3## wherein R is selected from the group consisting of sodium, potassium, ammonium, 2 -amino-2-methyl 1,3-propanediol, dicyclohexylamine, cyclohexylamine and tris(hydroxymethyl) amminomethane.
A composition for the assay of alkaline phosphatase enzymes comprising a compound having the structure ##STR4## wherein R is selected from the group consisting of sodium, potassium, ammonium, 2 -amino-2-methyl 1,3-propanediol, dicyclohexylamine, cyclohexylamine and tris(hydroxymethyl) aminomethane, a phosphorylatable buffer in an amount sufficient to produce a pH of about 10.2 and an activator for alkaline phosphatase in an amount sufficient to activate said alkaline phosphatase.
A method of assaying alkaline phosphatase enzymes present in a sample comprising contacting said sample with a composition which releases a dyestuff at a measurable rate, said composition comprising a compound having the structure ##STR5## wherein R is selected from the group consisting of sodium, potassium, ammonium, 2-amino-2-methyl 1,3-propanediol, dicylohexylamine, cyclohexylamine and tris(hydroxymethyl) aminomethane, a phosphorylatable buffer in an amount sufficient to produce a pH of about 10.2 and an activator for alkaline phosphatase enzymes in an amount sufficient to activate said enzymes and spectrophotometrically measuring the rate of formation of dye.
A method of assaying alkaline phosphatase enzymes present in a sample comprising contacting said sample with a composition comprising a compound having the structure ##STR6## wherein R is selected from the group consisting of sodium, potassium, ammonium, 2-amino-2-methyl 1-3propanediol, dicyclohexylamine, cyclohexylamine and tris(hydroxymethyl) aminomethane, a phosphorylatable buffer in an amount sufficient to produce a pH of about 10.2 and an activator for alkaline phosphatase enzymes in an amount sufficient to activate said enzymes, adding alkali, and determining the absorbance of the resulting composition.
A method of assaying acid phosphatase enzyme present in a sample comprising contacting said sample with a composition comprising a compound having the structure ##STR7## wherein R is selected from the group consisting of sodium, potassium, ammonium, 2-amino-2-methyl 1,3- propanediol, dicyclohexylamine, cyclohexylamine and tris (hydroxymethyl) aminomethane, a buffer in an amount sufficient to produce an acid pH, adding alkali and determining the absorbance of the resulting composition.
Dependencies are inferred from the claim text.