Single sample method for determination of lipoprotein concentrations in blood
AssigneeOrtho Diagnostics, Inc.
InventorJohn William Teipel
Life of this patent
Estimating the term…
Abstract
A single-sample method for determining concentrations of individual lipoprotein classes and lipids in blood by turbidimetric measurement. The blood serum is mixed with an ionic-strength-sensitive agent for forming an insoluble complex with the chylomicron, very low density, and low density lipoprotein classes, and the ionic strength of the mixture is raised in steps to cause progressive dissolution of each class of complex from that of highest density lipoprotein to that of lowest density lipoprotein. Measurement of the turbidity due to the insoluble complex(es) present at each step allows calculation of the turbidity due to the complex of each individual lipoprotein class and from that the concentration of each lipoprotein class and lipid in the blood sample.
Claims20
A single-sample method for determining the concentrations of low density lipoprotein (LDL), very low density lipoprotein (VLDL) and chylomicrons (CHYLO) in blood which comprises:
- a. mixing the serum of said blood with an ionic-strength-sensitive agent for forming an insoluble complex with substantially all of said LDL, said VLDL, and said CHYLO, comprising a sulfated polysaccharide and the salt of a divalent cation whereby turbidity is produced in said mixture;
- b. measuring the turbidity of said mixture;
- c. increasing the ionic strength of the mixture formed in step a) to a value whereby the LDL complex dissolves, so that the remaining turbidity of said mixture is due primarily to the insoluble complexes of VLDL snd CHYLO;
- d. measuring said remaining turbidity of said mixture;
- e. increasing the ionic strength of the mixture formed in step c) to a value whereby the VLDL complex dissolves, so that the final turbidity of said mixture is due primarily to the insoluble complex of CHYLO;
- f. measuring said final turbidity of said mixture;
- g. and utilizing said turbidities to determine the concentration of LDL, VLDL, and chylomicrons.
A single-sample method for determining the concentrations of triglyceride and cholesterol in blood by forming insoluble complexes of low density lipoprotein (LDL), very low density lipoprotein (VLDL) and chylomicrons (CHYLO) which comprises:
- a. mixing the serum of said blood with an ionic-strength-sensitive agent for forming an insoluble complex with substantially all of said LDL, said VLDL, and said CHYLO comprising an aqueous solution of a sulfated polysaccharide and the salt of a divalent cation, whereby turbidity is produced in said mixture;
- b. measuring the turbidity of said mixture and determining the sum of the concentrations of triglyceride and cholesterol therefrom;
- c. increasing the ionic strength of the mixture formed in step a) to a value whereby the LDL complex dissolves, so that the remaining turbidity of said mixture is due primarily to the insoluble complexes of VLDL and CHYLO;
- d. measuring said remaining turbidity of said mixture and determining the concentration of triglyceride therefrom;
- e. subtracting the concentration of triglyceride obtained in step d from the sum of the concentrations of triglyceride and cholesterol obtained in step b to get the concentration of cholesterol.
A single-sample method for determining the concentrations of triglyceride and cholesterol in blood by forming insoluble complexes of low density lipoprotein (LDL), very low density lipoprotein (VLDL) and chylomicrons (CHYLO) which comprises;
- a. mixing the serum of said blood with an ionic-strength-sensitive agent for forming an insoluble complex with substantially all of said LDL, said VLDL, and said CHYLO comprising an aqueous solution of a sulfated polysaccharide and the salt of a divalent cation, whereby turbidity is produced in said mixture;
- b. measuring the turbidity of said mixture;
- c. increasing the ionic strength of the mixture formed in step a) to a value whereby the LDL complex dissolves, so that the remaining turbidy of said mixture is due primarily to the insoluble complexes of VLDL and CHYLO;
- d. measuring said remaining turbidity of said mixture and determining the concentration of triglyceride therefrom;
- e. subtracting the turbidity obtained in step d) from that obtained in step b) to obtain the turbidity due to the insoluble complex of LDL alone and determining the concentration of cholesterol therefrom.
A single-measurement method for rapidly determining the sum of the concentrations of triglyceride and cholesterol in blood by forming insoluble complexes of low density lipoprotein (LDL), very low density lipoprotein (VLDL) and chylomicrons (CHYLO) which comprises:
- a. mixing the serum of said blood with an ionic-strength sensitive agent for forming an insoluble complex with substantially all of said LDL, said VLDL, and said CHYLO comprising an aqueous solution of a sulfated polysaccharide and the salt of a divalent cation, whereby turbidity is produced in said mixture;
- and
- b. measuring the turbidity of said mixture and determining the sum of the concentrations of triglyceride and cholesterol therefrom.
Dependencies are inferred from the claim text.