Back to results
US 4,034,074 AGranted
Universal reagent 2-site immunoradiometric assay using labelled anti (IgG)
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorLaughton E. M. Miles
Life of this patent
Estimating the term…
Abstract
An improvement is made in 2-site immunoradiometric assay wherein a single labelled antibody can be used to assay many different antigens.
Claims3
The process of making an immunoradiometric quantitative assay of an unknown quantity of a known antigen of a first animal species comprising the steps of:
- a. preparing an antibody to the antigen of the first animal species in a second animal species,
- b. causing said antibody to be bound to a reaction site,
- c. reacting and binding said known antigen with said bound antibody,
- d. preparing an antibody to said known antigen in a third animal species and reacting and binding the same with the bound known antigen of paragraph c,
- e. preparing a purified, radioactive labeled antibody to the immunoglobulin of said third animal species in said second animal species and reacting and binding the same with the bound material of paragraph d,
- f. removing unreacted material from said reaction site, and
- g. measuring the radioactivity of the bound material to determine the quantity of the known antigen.
4 more elements
Dependencies are inferred from the claim text.