Hepatitis A antigen
AssigneeMerck & Co., Inc.
InventorsPhilip J. Provost, Oswald L. Ittensohn, Maurice R. Hilleman
Life of this patent
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Abstract
Hepatitis A antigen obtained from the livers of non-human primates infected with hepatitis A (infectious hepatitis) virus has been found to be usable in fast, simple assays for hepatitis A antibody and thus usable in diagnosis of hepatitis A disease in humans, and for the preparation of hepatitis A vaccine.
Claims3
A method for obtaining hepatitis A antigen in an amount effective to complex hepatitis A antibody comprising inoculating a non-human primate with hepatitis A virus, subsequently removing the liver at a time when the serum glutamic oxaloacetic transaminase enzyme and the serum isocitric dehydrogenase enzyme levels are elevated, perfusing the liver with physiological saline, comminuting the liver and adding saline to obtain a suspension containing from about 5% to about 25% by weight of liver, clarifying the suspension, treating the suspension with CsCl gradient and selecting material having a buoyant density of from about 1.32 g/cm.sup.3 to about 1.36 g/cm.sup.3 of CsCl.
Dependencies are inferred from the claim text.