Back to results
US 4,029,549 AGranted
Process of producing 9.alpha.-hydroxy-3-ketobisnorchol-4-en-22-oic with mycobacterium fortuitum
AssigneeThe Upjohn Company
InventorsFrederick J. Antosz, Willard J. Haak, Merle G. Wovcha
Life of this patent
Estimating the term…
Abstract
The novel compound 9.alpha.-hydroxy-3-ketobisnorchol-4-en-22-oic, hereinafter referred to as 9.alpha.-OH BN acid. This compound is producible in a fermentation using the microorganism Mycobacterium fortuitum, NRRL B-8119. 9.alpha.-OH BN acid can be used as an intermediate to make useful steroids.
Claims15
A process for preparing 9.alpha.-hydroxy BN acid which comprises:
- a. cultivating a 9.alpha.-hydroxy BN acid producing microorganism in an aqueous nutrient medium under aerobic conditions in the presence of a steroid containing from 8 to 10 carbon atoms, inclusive, in the 17-alkyl side chain to produce 9.alpha.-hydroxyandrostenedione and 9.alpha.-hydroxy BN acid in the fermentation beer, and,
- b. isolating said 9.alpha.-hydroxy BN acid free from 9.alpha.-hydroxyandrostenedione.
A process for preparing 9.alpha.-hydroxy BN acid which comprises:
- a. cultivating Mycobacterium fortuitum NRRL B-8119 in an aqueous nutrient medium under aerobic conditions in the presence of a steroid containing from 8 to 10 carbon atoms, inclusive, in the 17-alkyl side chain to produce 9.alpha.-hydroxyandrostenedione and 9.alpha.-hydroxy BN acid in the fermentation beer,
- b. filtering said fermentaton beer to give a filtrate,
- c. extracting said filtrate with butyl acetate at a pH of about 4.0 to give an extract,
- d. washing said extract with a 5% sodium bicarbonate solution to give a wash solution,
- e. extracting said wash solution with butyl acetate to give an extract, and
- f. crystallizing 9.alpha.-hydroxy BN acid from said extract by concentration of the extract.
3 more elements
Dependencies are inferred from the claim text.