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US 4,021,303 AGranted
Method for treatment of microorganisms
AssigneeDai-Nippon Sugar Manufacturing Co., Ltd.
InventorYutaka Nakabayashi
Life of this patent
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Abstract
Microorganisms are chemically treated with alkali at a pH of 10.5 - 12.9 and a temperature of 0.degree. - 30.degree. C., washed with water and mechanically ruptured at a pH of 7 - 10.2.
Claims7
A method for treating a slurry of microorganisms which comprises
- 1. chemically treating the microorganisms for at least about 4 minutes with at least one alkali selected from the group consisting of sodium hydroxide and potassium hydroxide at a pH of from 10.5 to 12.9 and at a temperature of from 0.degree. C. to 30.degree. C. to form a mixture of chemically treated microorganism cells and waste liquid,
- 2. separating said microorganism cells from said waste liquid,
- 3. washing the separated microorganism cells with water to remove eluted nucleic acids or substances related thereto and lipids,
- 4. adding water to form a slurry of microorganism cells,
- 5. mechanically rupturing the cell membranes of the resulting chemically treated microorganisms using a three roll mill, or a sand grinder of the slit separation type at a pH of 7 to 10.2.
2 more elements
Dependencies are inferred from the claim text.