Cell cultures
AssigneeBurroughs Wellcome Co.
InventorsSidney Edwin Smith, Kevin Joseph O'Reilly, John Prydie
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Abstract
Cell culture comprising a novel cell strain in suitable nutrient medium, wherein the novel cell strain is produced by disaggregated feline embryo tissue cell and serially passaging the same from about the 20th to about the 48th passage level, so as to be cultured and subcultured with the resultant cell strains remaining substantially diploid, typically fibroblastic and substantially free from morphological transformations. Subculturing is facilitated by treatment of a confluent sheet of the disaggregated cells chelating agent before each transfer into a new batch of nutrient medium.
Claims1
A cell culture comprising a cell strain and a suitable nutrient medium therefor, wherein said cell strain is a feline embryo cell strain produced by disaggregating by chemical or mechanical means feline embryo tissue cells susceptible to feline infectious enteritis virus and serially passaging said cells up to at least the 20th passage level and at most the 48th passage level in a suitable nutrient media, said nutrient medium having a pH between 6.8-7.8 and a temperature in the range of 32.degree. C. to 39.degree. C., so as to be cultured and sub-cultured, wherein said sub-culturing is facilitated by treating the confluent sheet of said cells with a chelating agent before each transfer into a new batch of suitable nutrient medium, said resultant cell strains remaining substantially diploid, typically fibroblastic, and substantially free from morphological transformations.
Dependencies are inferred from the claim text.