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US 4,021,117 AGranted
Process for automatic counting and measurement of particles
OwnerHildegard Gohde, Wolfgang Gohdeinventors
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Abstract
A method of counting and analyzing pulses representative of characteristics of particles passing through a detector device including detecting the amplitude of each pulse, measuring the area under each pulse and above a reference level, deriving a ratio of amplitude to measured area, and determining which pulses have a ratio over a selected threshold as an indication of simultaneous passage of more than one pulse through the sensing zone of the detector device.
Claims5
A method of automatic counting and measurement of particles, especially biological cells, suspended in a fluid medium, comprising
- passing the medium and the particles suspended therein through a physically defined zone;
- producing electrical signals having amplitudes and durations representative of characteristics of the particles passing through the zone;
- measuring the maximum amplitude of each of the signals;
- measuring the area under each of the signals and greater than a preselected level;
- determining the ratio of the measurements of maximum amplitude to area;
- and
- identifying those signals for which the ratio exceeds a predetermined value as being indicative of the coincidental passage of more than one particle through said zone.
4 more elements
Dependencies are inferred from the claim text.