Biological diagnostic test strip and method of producing same
AssigneeLachema, narodni podnik
InventorsVlastimil Svoboda, Olga Celchovska
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Abstract
Diagnostic test strips for the qualitative detection and semi-quantitative estimation of blood and hemoglobin in biological products include a reagent area comprising an acid buffer having a pH within the range of 2.5-5.0, a chromogen, a wetting agent, an agent capable of enhancing the peroxidase activity of hemoglobin, an organic hydroperoxide in the form of a stable, solid salt with an aliphatic, alicyclic or heterocyclic amine, and a solid, polymeric film forming material disposed upon an adsorbent bibulous carrier.
Claims7
Diagnostic test strip for the detection and semi-quantitative estimation of blood and hemoglobin in biological materials comprising a bibulous carrier impregnated with a composition of matter comprising (a) an acid buffer having a pH within the range of 2.5-5.0, (b) a chromogen, (c) an agent capable of enhancing the peroxidase activity of hemoglobin, (d) a wetting agent, (e) a solid, polymeric film-forming substance, and (f) an amine salt of an organic hydroperoxide.
Method for the preparation of a diagnostic test strip for the detection and semi-quantitative estimation of blood and hemoglobin in biological materials which comprises the steps of
- a. impregnating an adsorbent carrier with a solution of an acid buffer having a pH within the range of 2.5-5.0, and a chromogen,
- b. drying the resultant impregnated material at elevated temperatures,
- c. impregnating the dried carrier with a hydroperoxide salt comprising the reaction product of an organic hydroperoxide with an amine selected from the group consisting of aliphatic, alicyclic and heterocyclic amines having a pK of at least 8.0, and
- d. drying the resultant impregnated carrier.
Dependencies are inferred from the claim text.