Phenethylamine antigenic conjugates, their preparation, antibodies, and use
AssigneeBiological Developments, Inc.
InventorJames R. Soares
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Abstract
Antigenic phenethylamine conjugates are produced by blocking the amine of the phenethylamine hapten with a group, coupling of the phenethylamine to a carrier, and removal of the blocking group. Specific phenethylamines useful in this invention are amphetamine, epinephrine, and norepinephrine. The antigens thus produced can be used to raise antibodies in animal bodies, the antibodies being specific to the phenethylamine compound employed. In particular, this disclosure is directed to blocked phenethylamines for production of antigens and to a process for preparing the antigens.
Claims14
A process for producing a synthetic antigen of formula ##STR10## where R' and R" are individually selected from the class consisting of --H, --OH, and --OCH.sub.3, where either R' or R" is --OCH.sub.3, the other is --OH;
- R"' is selected from the class consisting of --H and --OH;
- R"" is selected from the class consisting of --H, --OCH.sub.3, and --C.sub.2 H.sub.5 ;
- Q is selected from the class consisting of --H and --CH.sub.3 ;
- Y is a linking group which is the residue of the reaction of a coupling agent with a reactive group derivatized into the phenyl ring and of the coupling agent with the carrier, and the carrier is a macromolecule conferring antigenicity;
- where said reactive group is and said Y group includes a -N = N- or a group having the formula ##STR11##
- where Q' is an alkylene chain having up to 20 carbon atoms;
- comprising 1. substituting a blocking group onto the amine of a phenylamine having the formula:
- ##STR12## to form an amine blocked phenethylamine;
Dependencies are inferred from the claim text.