Enzymatic immunological method for the determination of antigens and antibodies

A method for determining the presence of a component of an antigen-antibody reaction in a liquid sample containing the component to be determined comprising the steps of:

• a. providing a given quantity of a first reagent consisting of one component of said reaction selected from the group consisting of an antigen and an antibody bound to the surface of water-insoluble, water-insuspensible, solid carrier, and also providing a predetermined amount of the binding partner for the component to be determined when the component in said liquid sample and the component bound to said solid carrier have the same immunochemical properties;
• b. contacting and incubating a given quantity of said liquid sample with said first reagent;
• c. washing said solid carrier;
• d. providing a given quantity of a substance having the same immunological properties as the component previously bound to the solid carrier, said substance being covalently linked to an enzyme;
• e. contacting and incubating the solid phase from step (c) with said enzyme-linked substance;
• f. washing the solid carrer;
• and
• g. determining the enzyme activity substance bound to the solid phase, which is a measure of the presence and quantity of the component to be determined.

A method for the detection and determination of a component in the reaction of a hepatitis antigen and the associated antibody in a liquid sample containing said component, comprising the steps of:

• a. providing a given quantity of a reagent consisting of one component of said reaction selected from the group consisting of said antigen and said antibody bound to a water-insoluble, water-insuspensible, solid carrier;
• b. contacting and incubating said liquid sample with said solid carrier of step (a) to form a reaction mixture;
• c. providing a given quantity of a substance having the same immunological properties as the component bound to said solid carrier, said substance being covalently linked to an enzyme, and also providing a predetermined amount of the binding partner for the component to be determined when the component in said liquid sample and the component bound to said solid carrier have the same immunochemical properties;
• d. contacting and incubating the reaction mixture with said enzyme-linked substance;
• and
• e. determining the enzyme activity of substance bound to the solid phase, which is a measure of the presence and quantity of the component to be determined.

A diagnostic pack for the detection and determination of a component of an antigen-antibody reaction selected from the group consisting of an antigen and an antibody, consisting of:

• a. one of said components coupled to a water-insoluble, water-insuspensible, solid carrier;
• b. a substance having the same immunological properties as the component in (a) covalently linked to an enzyme;
• and
• c. the binding partner of the component to be determined if the component to be determined has the same immunological properties as the component in (a).

A diagnostic pack for the detection and determination of hepatitis B consisting of:

• a. the antibody against Hepatitis B surface antigen coupled to a water-insoluble, water-insuspensible, solid carrier;
• b. said antibody covalently linked to an enzyme;
• and
• c. Hepatitis B surface antigen in case the component to be determined is the antibody against Hepatitis antigen.