Process for producing .alpha.-1,6 glucosidases using thermophilic microorganisms
AssigneeStandard Brands Incorporated
InventorsR. Otto Horwath, John A. Lally, Philip Rotheim
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Abstract
Thermophilic microorganisms which produce .alpha.-1,6 glucosidases are grown under aerobic conditions in an aqueous nutrient medium containing suitable carbohydrate sources. The medium is maintained at a suitable pH and, typically, at a temperature above about 40.degree. C to provide substantial growth of the microorganisms and production of .alpha.-1,6 glucosidases.
Claims15
A process for producing .alpha.-1,6 glucosidases comprising growing thermophilic microorganisms selected from the group consisting of the Bacillus genus, the Antinomycetales order and gram negative rod shaped microorganisms which produce .alpha.-1,6 glucosidases under aerobic conditions in an aqueous nutrient medium having present a carbohydrate selected from the group consisting of glucose, isomers of glucose, oligomers and polymers containing a glucose moiety or isomers of a glucose moiety and mixtures thereof and maintaining the medium at a suitable pH and a temperature of from about 45.degree. to about 70.degree. C to provide substantial growth of the microorganisms and production of .alpha.-1,6 glucosidase enzymes.
A process for producing .alpha.-1,6 glucosidases as defined in claim 9, wherein the pH of the aqueous nutrient medium is maintained in the range of from about 6.4 to about 7.2 during growth of the microorganisms.
Dependencies are inferred from the claim text.