Microbiological reduction of zearalenone and related compounds
AssigneeIMC Chemical Group, Inc.
InventorJames Robert McMullen
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Abstract
A method for microbiologically reducing zearalenone and related compounds comprising cultivating a ketone-reducing microorganism in a nutrient medium and exposing the compound being reduced to the enzyme action of said microorganism.
Claims17
A method for reducing a keto-compound of the formula ##STR10## to a hydroxy-compound of the formula ##STR11## wherein R and R.sup.1 may be the same or different and each is selected from the group consisting of hydrogen, hydroxy, and --OR.sup.2 wherein R.sup.2 is selected from the group consisting of lower alkyl of from 1 to about 6 carbon atoms, monocyclic aryl of about 6 to 8 carbon atoms, and monocyclic aralkyl, in which the alkyl portion has 1 to about 5 carbon atoms and the aryl portion has about 6 to 8 carbon atoms;
- and Y is selected from the group consisting of --CH.sub.2 --CH.sub.2 -- and --CH=CH--, which comprises cultivating a ketone-reducing, enzyme-producing microorganism in a nutrient fermentation medium, said enzyme being capable of reducing the keto-group of said keto-compound, and contacting the keto-compound with the ketone-reducing enzyme produced by the microorganism to reduce the keto-compound to produce the hydroxy-compound.
A method for reducing a keto-compound of the formula ##STR12## to a hydroxy-compound of the formula ##STR13## wherein R and R.sup.1 may be the same or different and each is selected from the group consisting of hydrogen, hydroxy, and --OR.sup.2, wherein R.sup.2 is lower alkyl of from 1 to about 6 carbon atoms, monocyclic aryl of about 6 to 8 carbon atoms, and monocyclic aralkyl, in which the alkyl portion has 1 to about 5 carbon atoms and the aryl portion has about 6 to 8 carbon atoms;
- and Y is selected from the group consisting of --CH.sub.2 -- CH.sub.2 -- and --CH=CH--, which comprises inoculating a nutrient fermentation medium with a ketone-reducing, enzyme-producing microorganism, said enzyme being capable of reducing the keto-group of said keto-compound;
- incubating said nutrient fermentation medium under aerobic conditions until substantial cell concentration is achieved;
- introducing the keto-compound into the nutrient fermentation medium;
- and incubating said nutrient fermentation medium under substantially anaerobic conditions until substantial reduction of the keto-compound to the hydroxy-compound is achieved.
A method for reducing a keto-compound of the formula ##STR14## to a hydroxy-compound of the formula ##STR15## wherein R and R.sup.1 may be the same or different and each is selected from the group consisting of hydrogen, hydroxy, and --OR.sup.2, wherein R.sup.2 is selected from the group consisting of lower alkyl of from 1 to about 6 carbon atoms, monocyclic aryl of about 6 to 8 carbon atoms, and monocyclic aralkyl, in which the alkyl portion has 1 to about 5 carbon atoms and the aryl portion has about 6 to 8 carbon atoms;
- and Y is selected from the group consisting of --CH.sub.2 -- CH.sub.2 -- and --CH=CH--, which comprises contacting the keto-compound with an enzyme under ketone-reducing conditions to reduce the keto-compound to produce the hydroxy-compound, wherein said enzyme is capable of reducing the keto-group of said keto-compound and is produced by cultivating a microorganism capable of producing said enzyme in a nutrient fermentation medium.
Dependencies are inferred from the claim text.