Method and reagents for the detection, estimation and quantitative determination of nitrate ions
AssigneeBen-Gurion University of the Negev Research and Development Authority
InventorEugen Szekely
Life of this patent
Estimating the term…
Abstract
Reagent and method for the detection, estimation and quantitative determiion of nitrate ions by mixing the reagents with aqueous nitrate samples, the intensity of the color reactions which result being proportional to the nitrate content of the samples which can be precisely measured by photometric measurements or estimated by comparison with color standards.
Claims24
A method for the detection, estimation and quantitative determination of nitrate ions comprising preparing a nitrate reagent comprising:
- a. about 0.005 to 0.05 wt/v %. of a diphenylamine redox indicator;
- b. about 0.1 to 2.0 wt/v %. 4,4'-sulfonyldianiline;
- c. about 0.1 to 2.0 wt/v %. of a chloride selected from the group consisting of ammonium chloride, hydroxylamine hydrochloride or a mixture thereof;
- d. concentrated sulfuric acid;
- and
- e. concentrated phosphoric acid or an alkaline dihydrogen phosphate;
- and combining said reagent with an aqueous nitrate sample.
A reagent for the detection, estimation and quantitative determination of nitrate ions upon combination with concentrated sulfuric acid and concentrated phosphoric acid or an alkaline dihydrogen phosphate comprising
- a. about 0.005 to 0.05 wt/v %. of a diphenylamine redox indicator;
- b. about 0.1 to 2 wt/v %. of 4,4'-sulfonyldianiline, and
- c. about 0.1 to 2.0 wt/v %. of a chloride selected from the group consisting of ammonium chloride, hydroxylamine hydrochloride or a mixture thereof.
A reagent for the detection and estimation of nitrate ions upon combination with concentrated sulfuric acid comprising:
- a. about 0.005 to 0.05 wt/v %. of a diphenylamine redox indicator;
- b. about 0.1 to 2 wt/v %. of 4,4'-sulfonyldianiline;
- c. about 0.1 to 2.0 wt/v %. of a chloride selected from the group consisting of ammonium chloride, hydroxylamine hydrochloride or a mixture thereof;
- and
- d. an alkaline dihydrogen phosphate.
A reagent for the detection, estimation and quantitative determination of nitrate ions comprising:
- a. about 0.005 to 0.05 wt/v %. of a diphenylamine redox indicator;
- b. about 0.1 to 2.0 wt/v %. 4,4'-sulfonyldianiline;
- c. about 0.1 to 2.0 wt/v %. of a chloride selected from the group consisting of ammonium chloride, hydroxylamine hydrochloride or a mixture thereof.
- d. concentrated sulfuric acid;
- and
- e. concentrated phosphoric acid or an alkaline dihydrogen phosphate.
Dependencies are inferred from the claim text.