Affinity labelling enzymes with esters of aromatic sulfonic acids
AssigneeUnited States of America
InventorsShow-Chu Wong, Elliott Shaw
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Abstract
Novel esters of aromatic sulfonic acids are disclosed. The specific esters are nitrophenyl p- and m-amidinophenylmethanesulfonate. Also disclosed is a method for specific inactivation of the enzyme, thrombin, employing nitrophenyl p-amidinophenylmethanesulfonate.
Claims2
In a method for the study of enzymes which comprises affinity labelling enzymes for identifying or inhibiting their functional groups, the improvement which comprises reacting a purified enzyme with a compound selected from the group consisting of nitrophenyl p- and m-amidinophenylmethanesulfonate whereby there is obtained covalent bond formation between said compound and said enzyme with inactivation or inhibition of the enzyme.
Dependencies are inferred from the claim text.