Method of isomerizing glucose with enzyme immobilized within microbial cell
AssigneeDirector of National Food Research Institute
InventorsNobuzo Tsumura, Takafumi Kasumi
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Abstract
A method of isomerizing glucose with an enzyme immobilized within a microbial cell has been developed. The immobilized enzyme is prepared by dipping a microbial cell containing glucose isomerase in an aqueous solution containing citric acid and drying it, if necessary. An immobilization of an intracellular enzyme is reinforced by supplemental means such as radiation or others.
Claims22
A method of isomerizing glucose to fructose which comprises contacting an aqueous solution of glucose under conditions suitable for isomerization with a microbial cell having glucose isomerase immobilized therein, said microbial cell being prepared by dipping a microorganism containing glucose isomerase in an aqueous solution of citric acid or of a metal salt thereof, said metal being selected from the group of lithium, sodium, potassium, magnesium, cobalt, calcium and mixtures thereof.
Dependencies are inferred from the claim text.