Separation of proteins by hydrophobic adsorption
AssigneePalo Alto Medical Research Foundation
InventorBarend H. J. Hofstee
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Abstract
Separation of proteins from one another by passing an aqueous solution of a mixture of proteins through two or more bodies of matrices each having hydrophobic ligands covalently attached to it, the order of hydrophobicity being such that the protein mixture comes into contact with a first body having the least hydrophobic ligands, then with a second body having more hydrophobic ligands, etc., whereby the most highly hydrophobic proteins are adsorbed on the first (least hydrophobic) matrix, etc. in order of increasing hydrophobicity of the matrices and decreasing hydrophobicity of the proteins in the mixture.
Claims3
A method of separating two or more proteins from a mixture of proteins which comprises providing at least two bodies of hydrophobic adsorbent capable of adsorbing proteins by hydrophobic adsorption, said bodies having different hydrophobicities ranging from a lowest hydrophobicity to a highest hydrophobicity, and passing such protein mixture in aqueous solution through said bodies in order of increasing hydrophobicity whereby the proteins are bound to the bodies of adsorbent in order of hydrophobicities of the proteins ranging from a highest hydrophobicity to a lowest hydrophobicity.
Apparatus for adsorptive separation of proteins comprising a plurality of beds of adsorbent material each having the capacity to adsorptively bind to at least one protein, each bed having a hydrophobicity different from the hydrophobicity of the other beds, said beds being arranged in order of increasing hydrophobicity and being interconnected whereby when a solution containing a mixture of proteins passes through the beds they are preferentially adsorbed on successive beds in order of decreasing hydrophobicity.
Dependencies are inferred from the claim text.