Gradient separation apparatus
AssigneeE. I. Du Pont de Nemours and Company
InventorsWilliam A. Romanauskas, Oakley L. Weyant
Life of this patent
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Abstract
A centrifuge rotor has vertically oriented peripheral cavities and/or adaptors configured to receive sample containers each having a length which exceeds its diamter. Each cavity and/or adaptor is provided with a cap and/or an adaptor cap which together provide a rigid support and seal for each sample container. With this apparatus the separation gradient reorients from horizontal during separation to vertical during sample removal providing the advantages of reduced separation time and improved resolution.
Claims12
A centrifuge having a rotor with a vertically oriented spin axis for effecting horizontal gradient separations of a sample and vertical gradient reorientation of the separated sample components comprising, in combination:
- a plurality of elongated sample containers,
- housing means for mounting said containers about the spin axis of said rotor with the axis of each said container generally parallel to said spin axis,
- each said housing means being independent of said container and completely enclosing and providing a rigid support in all directions for a different said container particularly against vertically directed fluid pressure forces.
A centrifuge rotor for centrifuging an elongated sample container having an open end comprising in combination:
- a rotor having a vertically oriented rotational axis and defining a radially spaced, elongated cavity having a longitudinal axis generally parallel to said rotational axis,
- said cavity rigidly supporting said container and having an open end,
- a first cap sealing said open container end,
- a second cap secured to the open end of said cavity rigidly supporting said first cap.
A centrifuge rotor having a rotational axis, at least a single radially spaced elongated cavity defined by a bore closed at one end and a lip or counterbore each having a common axis substantially parallel to said rotational axis,
- said cavity adapted to rigidly support an open ended container,
- a first plug fitted in the open end of said bore for sealing the open end of a container, and
- a second plug secured in said counterbore for rigidly supporting said first plug and a container against axial movement.
Dependencies are inferred from the claim text.