Apparatus and method for measurement of total volume of particles in a liquid sample
AssigneeTechnicon Instruments Corporation
InventorsMorris H. Shamos, Michael J. Brand, William J. Calogero
Life of this patent
Estimating the term…
Abstract
Apparatus and method, utilizing an ion-selective electrode and a reference electrode, for determining the percentage volume of total particulates in a predetermined volume of a liquid medium or sample having an effective known concentration of a particular ion. The method includes introducing a predetermined volume of a diluent having a known concentration of that ion into the sample, and measuring with the electrodes the concentration of that ion in the diluted sample to indicate the percentage volume of total particulates in the sample. The apparatus and method for such determination are well suited for determining the percentage volume of total cells in a sample of whole blood.
Claims19
A method for determining the percentage total volume of particulates in a liquid sample, comprising the steps of:
- introducing a predetermined volume of a diluent having a known concentration of a particular ion into a predetermined volume of said sample having a known concentration of said particular ion, and determining the total liquid volume of said sample including measuring the concentration of said particular ion in the diluted sample.
A method for determining the percentage total volume of particulates in whole blood samples containing an anticoagulant and a known concentration of a particular ion, comprising the steps of:
- passing predetermined volumes of said samples along a first conduit, introducing a known volume of a diluent isotonic to blood into said conduit to be mixed with each of said samples, said diluent having a known concentration of said particular ion, and determining the total liquid volume of said sample including measuring the concentration of said ion in each sample-diluent mixture.
Apparatus for determining the percentage total volume of particulates in a liquid sample, comprising:
- means for mixing in sample-support means a predetermined volume of diluent having a known concentration of a particular ion and a predetermined volume of said sample having a known concentration of said particular ion, and means determining the total liquid volume of said sample including means measuring the concentration of said particular ion in said diluted sample.
Dependencies are inferred from the claim text.