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AssigneeThe United States of America as represented by the Department of Health, Education and Welfare
InventorsRobert M. Chanock, Brian R. Murphy
Estimating the term…
A method and resulting temperature sensitive (ts) recombinants which may be produced by modifying wild-type influenza A viruses by utilization of chemically mutagenized donor viruses possessing ts lesions and shut off temperatures in the area 37.degree.-38.degree. C. Such a recombinant virus has been produced that contains the surface H3 antigen of influenza A/Hong Kong/1968 (H3N2) virus and a temperature-sensitive (ts) lesion derived from a ts mutant of influenza A/Great Lakes/1965 (H2N2) virus. Additional donor viruses include influenza A/Hong Kong/1968-ts-1[A], influenza A double recombinant 10B, and influenza A/Hong Kong/1968-ts-1[E]. When utilized as a vaccine, these temperature-sensitive recombinant viruses have the facility to develop only mild symptoms, but the infection induces complete resistant to influenzal disease produced by challenge with a virulent wild-type virus. The temperature-sensitive donor mutant is prepared chemically by growth of the virus in the presence of 5-fluorouracil (5-FU).
A composition consisting of a virus and a compatible carrier wherein said virus consists of a temperature-sensitive (ts) recombinant mutant virus produced by growth of an influenza A virus in the presence of 5-fluorouracil to produce a chemically mutagenized and temperature-sensitive virus which is the donor of a defined genetic defect and which is mated with a virulent wild-type A virus of a different antigenic type to produce the recombinant mutant virus.
A method of producing a temperature-sensitive (ts) recombinant mutant virus which has a shut-off temperature of about 37.degree.-38.degree. C which comprises growing an influenza A virus in the presence of 5-fluorouracil to produce a donor virus possessing a temperature-sensitive genetic defect and mating said donor virus with a virulent wild-type A virus of a different antigenic type to produce a ts recombinant mutant virus of the new antigenic type.
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