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US 3,992,261 AGranted
Method for manufacture of maltose from starch by enzymes co-produced by a single microorganism
AssigneeAgency of Industrial Science and Technology
InventorsYoshiyuki Takasaki, Yoshimasa Takahara
Life of this patent
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Abstract
A microorganism which simultaneously produces .beta.-amylase and dextrin .alpha.-1,6-glucosidase is cultured. By use of the enzymes thus produced, starch is directly hydrolyzed into maltose in a high yield.
Claims9
A method for the simultaneous manufacture of .beta.-amylase and .alpha.-1,6-glucosidase from a microorganism, which comprises:
- culturing a microorganism selected from the group consisting of Bacillus cereus var. mycoides (ATCC No. 31102), Bacillus sp. YT 1002 (ATCC No. 31101) and Bacillus sp. YT 1003 (ATCC No. 31103) which belong to the genus Bacillus having the ability to produce .beta.-amylase and .alpha.-1,6-glucosidase simultaneously in a culture medium;
- and
- recovering the .sym.-amylase and .alpha.-1,6-glucosidase from the culture broth.
A method for the manufacture of maltose from starch or its derivatives with enzymes by a microoganism, which comprises:
- culturing a microoganism selected from the group consisting of Bacillus cereus var. mycoides (ATCC No. 31102), Bacillus sp YT 1002 (ATCC No. 31101) and Bacillus sp. YT 1003 (ATCC No. 31103) which belong to genus Bacillus having the ability to produce .beta.-amylase and .alpha.-1,6-glucosidase simultaneously in a culture medium;
- recovering said enzymes thus produced from the culture broth;
- adding said enzymes to said starch or its derivatives;
- and
- reating the resultant mixture under conditions such that said enzymes promote the hydrolysis of starch to maltose.
2 more elements
Dependencies are inferred from the claim text.