Substrate for the determination of desoxy-ribonuclease
AssigneeBehringwerke Aktiengesellschaft
InventorEkkehard Tiesler
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Abstract
A desoxy-ribonuclease substrate is prepared by boiling and homogenizing an aqueous dispersion of high molecular weight desoxy-ribonucleic acid (DNA) and 2-amino-7-dimethylamino-3-methyl diphenazthionium chloride (toluidine blue O) until a clear blue-colored solution is obtained containing a complex compound of the DNA and toluidine blue O. The substrate is used in tests for determination of desoxy-ribonuclease and it's antibodies.
Claims3
The method of making a solubilized complex compound of high-molecular-weight desoxy-ribonucleic acid (DNA) and 2-amino-7-dimethyl-amino-3-methyl-diphenazthionium chloride (toluidine blue O), which comprises boiling and homogenizing an aqueous dispersion of high-molecular weight DNA and toluidine blue O in a weight ratio of about 2:1, until a clear homogenous blue-colored solution is obtained.
Dependencies are inferred from the claim text.