Immobilization of enzymes with a starch-graft copolymer
AssigneeThe United States of America as represented by the Secretary of Agriculture
InventorsMary Ollidene Weaver, Edward B. Bagley, George F. Fanta, William M. Doane
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Abstract
Enzymes are immobilized by entrappment or by covalent bonding with alkali saponified gelatinized-starch-polyacrylonitrile graft polymers. The polymers absorb amounts of water equaling up to more than 1000 times their own weight. In addition to immobilizing enzymes, the polymers find many applications including their incorporation into products such as disposable diapers, surgical pads and sheets, and paper towels.
Claims11
A method of preparing an immobilized enzyme composition comprising:
- a. providing an aqueous-fluid-absorbing composition comprising water-insoluble alkali salts of aqueous alkali saponified gelatinized-starch-polyacrylonitrile graft polymers, which alkali salts were prepared by saponifying the product resulting from graft polymerization of acrylonitrile onto gelatinized starch, and which alkali salts contain gelatinized starch (GS) and saponified polyacrylonitrile (HPAN) in molar ratios of from about 1:1.5 to 1.9 GS:HPAN, said water-insoluble alkali salts of aqueous alkali saponified gelatinized-starch-polyacrylonitrile graft polymers being water-insoluble solids capable of absorbing in excess of 300 parts of water per part of said water-insoluble solids;
- b. mixing a solution of enzyme with the aqueous-fluid-absorbing composition whereby the solution of enzyme is absorbed into the fluid-absorbing composition thereby swelling said composition;
- and
- c. adding to said swollen composition an amount of water-soluble mineral salts sufficient to shrink the composition to an extent sufficient to entrap the enzyme within its matrices.
A method of preparing an immobilized enzyme composition comprising:
- a. providing an aqueous-fluid-absorbing composition comprising water-insoluble alkali salts of aqueous alkali saponified gelatinized-starch-polyacrylonitrile graft polymers, which alkali salts were prepared by saponifying the product resulting from graft polymerization of acrylonitrile onto gelatinized starch, and which alkali salts contain gelatinized starch (GS) and saponified polyacrylonitrile (HPAN) in molar ratios of from about 1:1.5 to 1.9 GS:HPAN, said water-insoluble alkali salts of aqueous alkali saponified gelatinized-starch-polyacrylonitrile graft polymers being water-insoluble solids capable of absorbing in excess of 300 parts of water per part of said water-insoluble solids;
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- b. mixing a solution of the enzyme and a coupling reagent with the fluid-absorbing composition thereby covalently bonding said enzyme to said composition and simultaneously swelling said composition.
Dependencies are inferred from the claim text.