Bonded carbohydrate stationary phases for chromatography
AssigneePurdue Research Foundation
InventorFrederick E. Regnier
Life of this patent
Estimating the term…
Abstract
A carbohydrate bonded support for use in a chromatographic system, and a method for preparation of such a support is disclosed herein. An inorganic support has a thin layer of carbohydrate or carbohydrate derivative covalently linked to the surface so that the thus formed support has excellent mechanical stability as well as the ability to avoid adsorbing or denaturing sensitive biological compounds, the carbohydrate bonded support thus combining the advantages of known supports without also incorporating the disadvantages thereof.
Claims21
A process for producing a bonded support for a chromatographic system, said process comprising:
- providing a porous inorganic support structure having available surface hydroxyl groups, said support structure having dimensions suitable for use as a support in a chromatographic system;
- bonding an organo silane onto said inorganic support structure;
- and
- covalently bonding a thin layer of carbohydrate material of less than about 30 A onto said organo silane whereby the formed support is mechanically stable with carbohydrate separation characteristics and is hydrophilic.
A process for producing a bonded support for a chromatographic system, said process comprising:
- providing a porous inorganic support structure having available surface hydroxyl groups, said support structure having dimensions suitable for use as a support in a chromatographic system;
- and
- covalently bonding a thin layer of glycidoxyl-propyl silane of less than about 30 A onto said inorganic support structure whereby the formed support is mechanically stable with good separation characteristics and is hydrophilic.
A process for producing a bonded support for a chromatographic system, said support comprising:
- providing a porous alkyl amine treated support structure having dimensions suitable for use in a chromatographic systems;
- and
- covalently bonding a thin layer of carbohydrate material of less than about 30 A onto an organo silane which is in turn bonded onto said alkyl amine treated support to thereby form a mechanically stable support that has the separation characteristics of carbohydrates and is hydrophilic.
Dependencies are inferred from the claim text.