Integral element for the analysis of cholesterol

An integral element for analysis of total cholesterol in a liquid, the element I comprising a spreading layer in fluid contact with a reagent layer and II containing

• a. a cholesterol ester hydrolyzing composition comprising lipase having cholesterol esterase activity and protease;
• and
• b. cholesterol oxidase;
• the cholesterol ester hydrolyzing composition and cholesterol oxidase being disposed within the element such that, in liquid applied to the element, cholesterol esters are saponified and cholesterol is decomposed producing a detectable change related to the total cholesterol content of the liquid, the spreading layer being capable of spreading within itself a substance selected from the group consisting of cholesterol, cholesterol esters or decomposition products of cholesterol to provide a uniform concentration of such substance at the surface of the spreading layer facing the reagent layer, and the reagent layer being of substantially uniform permeability to such substance or a reaction product of such substance.

An integral element for analysis of total cholesterol in a liquid, the element comprising a support upon which are superposed in fluid contact:

• 1. a spreading layer containing a cholesterol ester hydrolyzing composition comprising lipase having cholesterol esterase activity and protease;
• and
• 2. a reagent layer interposed between the spreading layer and the support and containing cholesterol oxidase and an indicator composition that can interact with a decomposition product of cholesterol to produce in the element a detectable change related to the total cholesterol content of the liquid
• the spreading layer being capable of spreading within itself a substance selected from the group consisting of cholesterol, cholesterol esters or decomposition products of cholesterol to provide a uniform concentration of such substance at the surface of the spreading layer facing the reagent layer, and the reagent layer being of substantially uniform permeability to such substance or a reaction product of such substance.

An integral element for analysis of total cholesterol in a liquid, the element comprising a support upon which are superposed in fluid contact;

• 1. a spreading layer containing (a) a cholesterol ester hydrolyzing composition comprising a lipase having cholesterol esterase activity and protease and (b) cholesterol oxidase;
• and
• 2. a reagent layer interposed between the spreading layer and the support and containing an indicator composition that can interact with a decomposition product of cholesterol to produce in the element a detectable change related to the total cholesterol content of the liquid
• the spreading layer being capable of spreading within itself a substance selected from the group consisting of cholesterol, cholesterol esters or decomposition products of cholesterol to provide a uniform concentration of such substance at the surface of the spreading layer facing the reagent layer, and the reagent layer being of substantially uniform permeability to such substance or a reaction product of such substance.

An integral element for analysis of total cholesterol in a liquid, the element comprising a support upon which are superposed in fluid contact:

• 1. a spreading layer containing a cholesterol ester hydrolyzing composition comprising lipase having cholesterol esterase activity and protease;
• 2. a reagent layer interposed between the spreading layer and the support and containing cholesterol oxidase and an indicator composition that can interact with a decomposition product of cholesterol to produce in the element a detectable change quantitatively related to the total cholesterol content of the liquid;
• and
• 3. interposed between the spreading layer and the reagent layer a barrier layer which inhibits passage of protease to the reagent layer.

An integral element for analysis of total cholesterol in a liquid, the element comprising in fluid contact:

• A. a spreading layer comprising blushed cellulose acetate;
• and
• B. a reagent layer comprising a hydrophilic colloid
• the element containing
• 1. a cholesterol ester hydrolyzing composition comprising lipase having cholesterol esterase activity and protease;
• and
• 2. cholesterol oxidase
• the cholesterol ester hydrolyzing composition and cholesterol oxidase being disposed within the element such that, in liquid applied to the element, cholesterol esters are saponified and cholesterol is decomposed producing a detectable change related to the total cholesterol content of the liquid.