Radioimmunoassay for determining the digoxin content of a sample
AssigneeAbbott Laboratories
InventorsRaymond Oslapas, Thomas Raymond Herrin
Life of this patent
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Abstract
A competitive radioimmunoassay for the measurement of digoxin in a serum sample in which the non-radioactive digoxin competes with a constant amount of a radioactively labeled digoxin tyramine analog comprising 3-O-(4-Hydroxyphenethylcarbamoyl) digoxigenin for binding cites on a limited amount of digoxin antibody. The percentage of the radioactively labeled digoxin which is bound to the antibody is inversely proportional to the concentration of digoxin in the serum sample. The concentration of digoxin in the serum is determined by the comparison with standards containing measured amounts of unlabeled digoxin.
Claims7
A method of measuring the digoxin content of a serum sample which comprises the steps of:
- a. adding digoxin antibody and a tracer amount of radioactively labeled 3-0-(4-Hydroxyphenethylcarbamoyl) digoxigenin reagent to said serum sample to form a mixture;
- b. incubating said mixture to permit the digoxin in the sample and said radioactively labeled reagent to bind to the digoxin antibody;
- c. adding a precipitating agent to the mixture to facilitate the formation of a precipitate and thereby separate the bound labeled digoxin from the free digoxin;
- d. separating the supernatant liquid from the precipitate which is formed;
- and
- e. measuring the radioactivity remaining in the precipitate.
A compound selected from the group consisting of 3-Chloroformyldigoxigenin and 3-0-(4-Hydroxyphenethylcarbamoyl) digoxigenin.
The compound 3-0-(4-Hydroxyphenethylcarbamoyl) digoxigenin.
Dependencies are inferred from the claim text.