US 3,981,772 A · Method of attenuating viruses with simultaneous stabilization of their antigens using selected aminomethylol compounds

Method of attenuating viruses with simultaneous stabilization of their antigens using selected aminomethylol compounds

OwnerAlexandr Mikhailovich Poverenny, Jury Alexeevich Semin +5inventors

InventorsAlexandr Mikhailovich Poverenny, Jury Alexeevich Semin, Mikhail Petrovich Chumakov, Antonina Vasilievna Gagarina, Maria Kronidovna Khanina, Evgeny Alexandrovich Tkachenko, Vyacheslav Nikolaevich Bashkirtsev

Life of this patent

Estimating the term…

Abstract

A method of attenuating viruses with a simultaneous stabilization of their antigens which consists in inactivating viruses with an aminomethylol compound having the formula ##EQU1## where R' is H, CH.sub.2 OH, COOH; R.sup.2 is H,(CH.sub.2).sub.n CH.sub.3, (CH.sub.2).sub.n NH.sub.2, (CH.sub.2).sub.n COOH or (CH.sub.2).sub.n OH; and N is an integer of 1-4, Said compound being in a concentration of from 6.6.times.10.sup.-.sup.3 to 1.6.times.10.sup.-.sup.3 mole at a temperature from 4.degree. to 32.degree.C is disclosed. This method can be used to prepare standard and reliable prophylactic attenuated viral vaccines and diagnostic preparations especially useful against dangerous viral diseases.

Claims2

Dependent claims

A method of attenuating the infectiousness of viruses with a simultaneous stabilization of their antigens which consists of attenuating a liquid virus-containing material with an aminomethylol derivative having the formula:

##EQU2## where:
R' is H, CH.sub.2 OH or COOH;
R.sup.2 is H, (CH.sub.2).sub.2 CH.sub.3, (CH.sub.2).sub.n NH.sub.2, (CH.sub.2).sub.n COOH or (CH.sub.2).sub.n OH;
and
N is an integer of 1 - 4
said compound being in a concentration of from 6.6.times.10.sup..sup.-3 to 1.6.times.10.sup..sup.-3 M at a temperature from 4.degree. to 32.degree.C, the selection of the temperature depending on the nature of the virus, and the exposure being sufficient for a complete attenuation of viral infectiousness.

3 more elements

Dependencies are inferred from the claim text.