Process for conditioning bacterial cells containing glucose isomerase activity
AssigneeMiles Laboratories, Inc.
InventorRaymond Lee Snell
Life of this patent
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Abstract
Bacterial cells having glucose isomerase activity are properly conditioned prior to use to produce a fructose-containing product by (a) mixing the cells with water or an aqueous dextrose solution under controlled conditions until the cells are properly hydrated and pH equilibrated and then (b) passing water or an aqueous dextrose solution up through a bed of such cells under controlled conditions until the effluent solution is clear and is stabilized at desired characteristics. When water is used in step (b), it is followed by the step of passing an aqueous dextrose solution up through the bed until the effluent solution has the same dextrose content as the entering dextrose solution.
Claims6
A process for conditioning bacterial cells having glucose isomerase activity for subsequent use to produce a fructose-containing product which comprises
- a. mixing the bacterial cells at about 10.degree.-27.degree.C. with water or an aqueous dextrose solution at a pH of about 8 measured at 25.degree.C. until the cells are hydrated and pH equilibrated;
- and
- b. passing an aqueous dextrose solution up through a bed of such bacterial cells at a temperature of about 60.degree.C. and at a pH of 7.5-8.0 measured at 60.degree.C. until the effluent solution is clear and the pH thereof is stabilized at 7.5-8.0 measured at 60.degree.C;
- or passing water at a temperature of about 10.degree.-27.degree.C. and at a pH of 8.0 measured at 25.degree.C. up through a bed of such bacterial cells until the effluent solution is clear and the pH thereof is stabilized at 8.0 measured at 25.degree.C., followed by the step of passing an aqueous dextrose solution up through the bed at a temperature of about 60.degree.C. and at a pH of 7.5-8.0 measured at 60.degree.C. until the effluent solution has the same dextrose content as the entering dextrose solution.
Dependencies are inferred from the claim text.