Blood control standard
OwnerAllan L. Louderbackinventor
Life of this patent
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Abstract
A stable blood control standard and method is provided for the quality control of the measurement of blood pH and gases in the clinical laboratory. The blood control standard comprises a sealed receptacle containing specially treated red cells and a gaseous head space at least equal to about the volume of the red cells. The special treatment comprises thorough washing and separating the red cells from the plasma components and mild treatment with aldehyde and retention in a buffered solution. The head space comprises from 0-15% CO.sub.2, 0-25% O.sub.2 and the balance N.sub.2 and/or inert gas.
Claims9
A blood control standard for the quality control of the measurement of blood pH and gases comprising a sealed receptacle containing treated erythrocytes and a gaseous head space having a volume which is at least equal to about the volume of said erythrocytes, said erythrocytes being treated by thorough washing in saline solution, mild admixing with a solution of aldehyde and saline, thorough washing in saline solution, buffering to a pH of from about 7 to about 7.7 and admixing with bicarbonate ion to a pCO.sub.2 of from about 20 to about 55 mm Hg, said gaseous head space comprising from about 0% to about 15% CO.sub.2, from about 0% to about 25% O.sub.2 and the balance selected from the group consisting of N.sub.2 and inert gases and mixtures thereof.
The method of making a blood control standard for the quality control of the measurement of blood pH and gases comprising thoroughly washing erythrocytes in saline solution, mildly admixing with a solution of aldehyde and saline, thoroughly washing is saline solution, buffering to a pH of from about 7 to about 7.7, admixing with bicarbonate ion to a pCO.sub.2 of from about 20 to about 55 mm Hg, transferring to receptacles while leaving a gaseous head space of at least about equal to the volume of said erythrocytes, flushing said head space with a gas comprising from about 0% to about 15% CO.sub.2, from about 0% to about 25% O.sub.2 and the balance selected from the group consisting of N.sub.2 and inert gases and mixtures thereof, and immediately thereafter sealing said receptacles to form a gas tight closure.
Dependencies are inferred from the claim text.